inverted fluorescence microscope (Nikon)
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Nikon
inverted fluorescence microscope
Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 12050 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+fluorescence+microscope/ECLIPSE+Ti2/pmc13674266-129-41-45
Average 99 stars, based on 12050 article reviews
Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 12050 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+fluorescence+microscope/ECLIPSE+Ti2/pmc13674266-129-41-45
Average 99 stars, based on 12050 article reviews
inverted fluorescence microscope - by Bioz Stars,
2026-10
99/100 stars
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Herein, we synthesized a disulfide-bridged amphiphilic glycyrrhetinic acidindomethacin prodrug conjugate (GA-ss-IND), which could spontaneously self-assemble into uniform nanomicelles driven by hydrophobic interaction and van der Waals forces, as verified by molecular dynamics simulations. Fluorescence:Article Title: Redox-responsive and liver-targeted nanoplatform for paclitaxel delivery toward hepatocellular carcinoma therapy. Article Snippet: Conventional paclitaxel (PTX) chemotherapy for hepatocellular carcinoma (HCC) is severely restricted by insufficient tumor-targeting specificity, unsatisfactory therapeutic efficacy, and marked systemic toxicity.. To address these drawbacks, a redox-responsive and liver-targeted nanoplatform was designed for efficient PTX delivery in HCC therapy.. Herein, we synthesized a disulfide-bridged amphiphilic glycyrrhetinic acidindomethacin prodrug conjugate (GA-ss-IND), which could spontaneously self-assemble into uniform nanomicelles driven by hydrophobic interaction and van der Waals forces, as verified by molecular dynamics simulations. Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke. Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair. Article Title: Mechanism of luteolin in treating chronic heart failure by regulating HIF-1 signaling pathway-mediated mitochondrial damage to inhibit cardiomyocyte apoptosis Article Snippet: The next day, a fluorophore-conjugated secondary antibody (Servicebio, Wuhan, China) and DAPI nuclear counterstain (Servicebio, Wuhan, China) were added sequentially in the dark. .. Fluorescence images were captured using an Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer Article Snippet: .. The cells were then stained with anti-CRT primary antibody for 16 h at 4 °C, washed with PBS, and stained with a secondary antibody for 4 h. 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Article Snippet: Conventional paclitaxel (PTX) chemotherapy for hepatocellular carcinoma (HCC) is severely restricted by insufficient tumor-targeting specificity, unsatisfactory therapeutic efficacy, and marked systemic toxicity.. To address these drawbacks, a redox-responsive and liver-targeted nanoplatform was designed for efficient PTX delivery in HCC therapy.. Herein, we synthesized a disulfide-bridged amphiphilic glycyrrhetinic acidindomethacin prodrug conjugate (GA-ss-IND), which could spontaneously self-assemble into uniform nanomicelles driven by hydrophobic interaction and van der Waals forces, as verified by molecular dynamics simulations. Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke. Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair. Article Title: Mechanism of luteolin in treating chronic heart failure by regulating HIF-1 signaling pathway-mediated mitochondrial damage to inhibit cardiomyocyte apoptosis Article Snippet: The next day, a fluorophore-conjugated secondary antibody (Servicebio, Wuhan, China) and DAPI nuclear counterstain (Servicebio, Wuhan, China) were added sequentially in the dark. .. Fluorescence images were captured using an Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer Article Snippet: .. The cells were then stained with anti-CRT primary antibody for 16 h at 4 °C, washed with PBS, and stained with a secondary antibody for 4 h. Finally, the cells were stained with DAPI and washed for fluorescence imaging using an Article Title: Mechanistic study on curcumin alleviating hepatic cell injury in common carp ( Cyprinus carpio ) via regulation of mitochondrial function Article Snippet: Key reagents: Mitochondria Isolation Kit (Sigma-Aldrich); ATP Assay Kit (Beyotime); Reactive Oxygen Species Assay Kit (DCFH-DA, Beyotime); Trizol Reagent for total RNA extraction (Invitrogen); PrimeScript RT Reagent Kit for reverse transcription (TaKaRa); TB Green Premix Ex Taq II for realtime quantitative PCR (TaKaRa); Activity Assay Kits for mitochondrial respiratory chain complexes I, II, III, citrate synthase (CS), and succinate dehydrogenase (SDH) (Nanjing Jiancheng Bioengineering Institute). .. Major instruments: Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling. Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging. Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an Phase Contrast:Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke. Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair. Morphology:Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke. Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair. Staining:Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer Article Snippet: .. The cells were then stained with anti-CRT primary antibody for 16 h at 4 °C, washed with PBS, and stained with a secondary antibody for 4 h. Finally, the cells were stained with DAPI and washed for fluorescence imaging using an Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling. Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging. Imaging:Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer Article Snippet: .. The cells were then stained with anti-CRT primary antibody for 16 h at 4 °C, washed with PBS, and stained with a secondary antibody for 4 h. Finally, the cells were stained with DAPI and washed for fluorescence imaging using an Real-time Polymerase Chain Reaction:Article Title: Mechanistic study on curcumin alleviating hepatic cell injury in common carp ( Cyprinus carpio ) via regulation of mitochondrial function Article Snippet: Key reagents: Mitochondria Isolation Kit (Sigma-Aldrich); ATP Assay Kit (Beyotime); Reactive Oxygen Species Assay Kit (DCFH-DA, Beyotime); Trizol Reagent for total RNA extraction (Invitrogen); PrimeScript RT Reagent Kit for reverse transcription (TaKaRa); TB Green Premix Ex Taq II for realtime quantitative PCR (TaKaRa); Activity Assay Kits for mitochondrial respiratory chain complexes I, II, III, citrate synthase (CS), and succinate dehydrogenase (SDH) (Nanjing Jiancheng Bioengineering Institute). .. Major instruments: Cell Culture:Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an Confocal:Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an |